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April 5, 2024

Documentation and Downloadable Files

All of the Streptococcus Laboratory resources and protocols available for download on this website are listed below.
The resources relate to S. pyogenes, S. agalactiae, S. pneumoniae or other catalase-negative, Gram-positive cocci.
They include DNA extraction, detection, and typing protocols, including for polymerase chain reaction (PCR)-based methods, as well as protocols for carriage studies.

DNA extraction protocols

Fast extraction of DNA for streptococcal culture isolates

Protocol for DNA extraction from bacterial isolates or clinical specimens with PCR tests

Blood and body fluid DNA extraction for streptococci

Protocol for DNA extraction from blood and body fluid using any type of PCR testing

Conventional PCR protocols

Conventional multiplex primers (pneumococcus)

Use for pneumococcal serotype deduction

emm typing protocol conventional PCR and sequencing

Detailed protocol for conventional polymerase chain reaction (PCR) and sequencing methods

Real-time PCR protocols

Singleplex targets

Use for S. pyogenes, S. pneumoniae, S. agalactiae, S. salivarius, and S. suis detection...

Triplex (S. pyogenes, S. agalactiae, and S. suis) targets

Use for S. pyogenes, S. agalactiae, and S. suis detection, as well as other pathogens...

Quadriplex targets (group A Streptococcus)

Use for emm typing of S. pyogenes

Quadriplex targets (pneumococcus)

Use for pneumococcal serotype deduction

Multiplex targets (pneumococcus antibiotic resistance and pili genes)

Use for detection of antibiotic resistance and pili genes of pneumococcal isolates or clinical speci...

emm typing resources

emm and emm-like genes

Documentation of multiple emm types being deduced from whole-genome sequence data

Distribution of emm types

List of emm types by emm cluster

emm list (alphabetic)

Documentation of converting st to emm, sorted alphabetically

emm list (numeric)

Documentation of converting st to emm, sorted numerically

Protocols for carriage studies

Identification and serotyping of pneumococci

Protocol for specimen collection, transport, and storage

Pneumococcus carriage study protocol

Protocol for nasopharyngeal swab processing

Alternative multilocus sequencing typing primers

Alternative multilocus sequencing typing primers

Primers for obtaining first few bases of the target sequence for S. pyogenes and S. pneumoniae...

April 5, 2024

Sources Print Share

Content Source:

National Center for Immunization and Respiratory Diseases; Division of Bacterial Diseases

Strep Lab

CDC's Streptococcus Laboratory is a reference center for pathogens that cause invasive streptococcal disease (groups A and B streptococci and pneumococcus).

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