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The National Institute for Occupational Safety and Health (NIOSH)
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The National Institute for Occupational Safety and Health (NIOSH)
1. Registry of Toxic Effects of Chemical Substances (RTECS)
Sulfuric acid, dimethyl ester
Related Pages
RTECS #
WS8225000
CAS #
77-78-1
See: NMAM or OSHA Methods
Updated
March 2019
Molecular Weight
126.14
Molecular Formula
C2H6O4S
Synonyms
Dimethyl monosulfate
Dimethyl sulfate
Dimethyl sulfate (ACGIH:OSHA)
Dimethyl sulphate
Dimethylester kyseliny sirove (Czech)
Dimethylsulfaat (Dutch)
Dimethylsulfat (Czech)
Dimetilsolfato (Italian)
DMS (methyl sulfate)
Dwumetylowy siarczan (Polish)
Methyl sulfate
Methyle (sulfate de) (French)
RCRA waste number U103
Sulfate de methyle (French)
Sulfate dimethylique (French)
Skin and Eye Irritation and References
Route/Organism Dose Effect Reference
eye /rabbit 100 mg/4S rinse severe FCTOD7 20,573,1982
eye /rabbit 50 µg/24H severe 85JCAE -,1079,1986
skin /rabbit 10 mg/24H open irritation test severe AMIHBC 4,119,1951
Mutation Data and Reference
System Test Route/Organism/Tissue Dose Reference
body fluid assay ovary/rat 300 mg/kg MUREAV 143,263,1985
Cytogenetic Analysis intraperitoneal/rat 350 mg/kg NASLDX 3,187,1980
Cytogenetic Analysis inhalation/rat 290 µg/m3/16W GTPZAB 30(9),38,1986
Cytogenetic Analysis unreported route/human 2 mg/m3 MUREAV 147,301,1985
Cytogenetic Analysis inhalation/mouse 240 µg/m3/10W GTPZAB 30(9),38,1986
Cytogenetic Analysis Ascites tumor/mouse 10 mmol/L BIZNAT 88,49,1970
Cytogenetic Analysis lung/hamster 50 µmol/L CRNGDP 3,385,1982
DNA adduct Ascites tumor/mouse 5 µmol/L MUREAV 89,95,1981
DNA adduct intraperitoneal/mouse 30 mg/kg CALEDQ 146,155,1999
DNA adduct inhalation/rat 0.1 ppm/6H INHTE5 16,581,2004
DNA adduct HeLa cell/human 5 µmol/L MUREAV 89,95,1981
DNA adduct /Escherichia coli 5 µmol/L MUREAV 89,95,1981
DNA Damage /Other microorganisms 100 mmol/L CBINA8 14,101,1976
DNA Damage /Other microorganisms 200 mg/L CBINA8 13,151,1976
DNA Damage /Bacillus subtilis 130 mmol/L MUREAV 9,141,1970
DNA Damage lymphocyte/human 1 mmol/L JACTDZ 1(3),125,1982
DNA Damage fibroblast/human 100 µmol/L CRNGDP 4,559,1983
DNA Damage liver/rat 100 µmol/L/3H MUREAV 629,49,2007
DNA Damage /Saccharomyes cerevisiae 13.3 mg/L/17H MUREAV 633,80,2007
DNA Damage intraperitoneal/mouse 100 mg/kg MUREAV 467,83,2000
DNA Damage intravenous/rat 80 mg/kg MUREAV 75,63,1980
DNA Damage liver/rat 30 µmol/L MUREAV 189,69,1987
DNA Damage inhalation/mouse 20 µg/kg PCBPBS 6,101,1976
DNA Damage lung/hamster 800 µmol/L/1H-continuous CRNGDP 1,795,1980
DNA inhibition oral/mouse 20 gm/kg ARGEAR 51,605,1981
DNA inhibition intraperitoneal/mouse 100 mg/kg MUREAV 46,305,1977
DNA inhibition HeLa cell/human 2 mmol/L MUREAV 92,427,1982
DNA inhibition fibroblast/human 75 µmol/L CRNGDP 4,559,1983
DNA repair /Escherichia coli 331 µmol/L MUREAV 272,145,1992
DNA repair /Other microorganisms 640 µg/plate BIZNAT 95,463,1976
gene conversion and mitotic recombination /Saccharomyes cerevisiae 1900 µmol/L SOGEBZ 17,915,1981
host-mediated assay Salmonella typhimurium/mouse 2500 µmol/kg CBINA8 19,241,1977
micronucleus test ovary/hamster 58.5 mg/L/24H MUREAV 633,80,2007
micronucleus test intraperitoneal/mouse 12500 µg/kg/48H- intermittent MUREAV 389,3,1997
mutation in mammalian somatic cells ovary/hamster 20 µmol/L MUREAV 57,217,1978
mutation in mammalian somatic cells lung/hamster 1 mg/L/1H CRNGDP 1,175,1980
mutation in microorganisms /Schizosaccharomyces pombe 590 µmol/L (-enzymatic activation step) CBINA8 14,195,1976
mutation in microorganisms /Neurospora crassa 1000 ppm/13M (-enzymatic activation step) GENTAE 67,189,1971
mutation in microorganisms /Saccharomyes cerevisiae 500 ppm/12M (-enzymatic activation step) GENTAE 78,1101,1974
mutation in microorganisms /Other microorganisms 1000 ppm/13M (-enzymatic activation step) GENTAE 67,189,1971
mutation in microorganisms /Other microorganisms 250 ppm (-enzymatic activation step) SOGEBZ 8,412,1972
mutation in microorganisms /Other microorganisms 2 mmol/L (-enzymatic activation step) HEREAY 46,279,1960
mutation in microorganisms /Salmonella typhimurium 10 µmol/plate (+enzymatic activation step) CBINA8 19,241,1977
mutation in microorganisms /Salmonella typhimurium 4300 nmol/L/1H (-enzymatic activation step) PNASA6 75,4465,1978
mutation in microorganisms /Escherichia coli 20 µmol/L (-enzymatic activation step) ARTODN 46,277,1980
other mutation test systems /Escherichia coli 2 nmol/tube PNASA6 79,5971,1982
other mutation test systems /Salmonella typhimurium 38 mg/L MUREAV 192,239,1987
other mutation test systems /Other microorganisms 100 mmol/L CBINA8 14,101,1976
phage inhibition capacity /Escherichia coli 65 mmol/L PRLBA4 150,497,1959
sex chromosome loss and nondisjunction oral/Drosophila melanogaster 3800 µmol/L NATUAS 203,1404,1964
sister chromatid exchange lung/hamster 10 µmol/L CRNGDP 3,385,1982
sister chromatid exchange fibroblast/human 1 µmol/L MUREAV 75,63,1980
specific locus test oral/Drosophila melanogaster 5 mmol/L MUREAV 182,243,1987
Unscheduled DNA Synthesis lymphocyte/human 10 µmol/L CRNGDP 13,1285,1992
Unscheduled DNA Synthesis fibroblast/human 50 µmol/L CRNGDP 4,559,1983
Unscheduled DNA Synthesis HeLa cell/human 400 µmol/L MUREAV 139,155,1984
Unscheduled DNA Synthesis lung/hamster 40 mg/L CBINA8 15,117,1976
Unscheduled DNA Synthesis liver/rat 100 µmol/L ENMUDM 3,11,1981
Reproductive Effects Data and References
Route/Organism Dose Effect Reference
intravenous/rat 100 mg/kg (15D pregnant) Reproductive: Effects on embryo or fetus: Fetal death IARCCD 4,45,1973
Tumorigenic Data and References
Route/Organism Dose Effect Reference
Tumorigenic: Equivocal tumorigenic agent by RTECS criteria
inhalation/rat lowest published toxic concentration: 17 mg/m3/19W- intermittent Olfaction: Tumors ZEKBAI 74,241,1970
Blood: Lymphoma including Hodgkin's disease
Tumorigenic: Carcinogenic by RTECS criteria
intravenous/rat lowest published toxic dose: 20 mg/kg (15D pregnant) Reproductive: Tumorigenic effects: Transplacental tumorigenesis IARCCD 4,45,1973
Brain and Coverings: Tumors
Tumorigenic: Equivocal tumorigenic agent by RTECS criteria
subcutaneous/rat lowest published toxic dose: 50 mg/kg ZEKBAI 74,241,1970
Tumorigenic: Tumors at site of application
Acute Toxicity Data and References
Route/Organism Dose Effect Reference
In Vitro/Human, monocyte Inhibitor Concentration (20 percent kill): 78 µmol/L/24H In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. TOLED5 199,51,2010
In Vitro/Human, monocyte Inhibitor Concentration (20 percent kill): 78 µmol/L/2H In Vitro Toxicity Studies: Other assays TOLED5 199,51,2010
inhalation/guinea pig lethal concentration (50 percent kill): 32 ppm/1H 85JCAE -,1079,1986
inhalation/human Lowest published lethal concentration: 97 ppm/10M 34ZIAG -,226,1969
Olfaction: Other olfaction effects
inhalation/mouse lethal concentration (50 percent kill): 280 mg/m3 Behavioral: Somnolence (general depressed activity) GTPZAB 23(3),28,1979
Lung, Thorax, or Respiration: Respiratory depression
Lung, Thorax, or Respiration: Dyspnea
inhalation/rat lethal concentration (50 percent kill): 45 mg/m3/4H Lung, Thorax, or Respiration: Cyanosis GTPZAB 24(11),55,1980
Blood: Hemorrhage
intravenous/rabbit lowest published lethal dose: 50 mg/kg Behavioral: Muscle contraction or spasticity AEXPBL 47,113,1902
oral/mouse lethal dose (50 percent kill): 140 mg/kg GTPZAB 23(3),28,1979
oral/rabbit lowest published lethal dose: 45 mg/kg Behavioral: Food intake (animal) AEXPBL 47,113,1902
oral/rat lethal dose (50 percent kill): 205 mg/kg GTPZAB 23(3),28,1979
Behavioral: Muscle contraction or spasticity
subcutaneous/rabbit lowest published lethal dose: 53 mg/kg AEXPBL 47,113,1902
Skin: After systemic exposure: Dermatitis, other
subcutaneous/rat lethal dose (50 percent kill): 100 mg/kg ZEKBAI 74,241,1970
Other Multiple Dose Data and References
Route/Organism Dose Effect Reference
inhalation/rat lowest published toxic concentration: 1.5 ppm/5D- intermittent Nutritional and Gross Metabolic: Weight loss or decreased weight gain INHTE5 16,581,2004
Olfaction: Ulcerated nasal septum
inhalation/rat lowest published toxic concentration: 0.7 ppm/2W- intermittent Olfaction: Other olfaction effects INHTE5 16,581,2004
Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
Reviews
Organization Standard Reference
American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value-time-weighted average 0.1 ppm (skin) DTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH) Threshold Limit Value-Confirmed animal carcinogen DTLVS* TLV/BEI,2013
International Agency for Research on Cancer (IARC) Cancer Review:Animal Sufficient Evidence IMEMDT 4,271,1974
International Agency for Research on Cancer (IARC) Cancer Review:Animal Sufficient Evidence IMEMDT 71,575,1999
International Agency for Research on Cancer (IARC) Cancer Review:Human Inadequate Evidence IMEMDT 4,271,1974
International Agency for Research on Cancer (IARC) Cancer Review:Human Inadequate Evidence IMEMDT 71,575,1999
International Agency for Research on Cancer (IARC) Cancer Review:Group 2A IMEMDT 71,575,1999
TOXICOLOGY REVIEW 85CVA2 5,63,1970
TOXICOLOGY REVIEW MUREAV 543,201,2003
TOXICOLOGY REVIEW CHHSA* 9,6,2002
TOXICOLOGY REVIEW FCTOD7 44,1699,2006
TOXICOLOGY REVIEW MUREAV 721,27,2011
TOXICOLOGY REVIEW MUTAEX 25,3,2010
TOXICOLOGY REVIEW MUREAV 763,294,2015
TOXICOLOGY REVIEW RTOPDW 54,247,2009
TOXICOLOGY REVIEW MUTAEX 33,179,2018
Standards and Regulations
Organization Standard Reference
Mine Safety and Health Administration (MSHA) STANDARD-air time-weighted average 1 ppm (5 mg/m3) (skin) DTLVS* 3,92,1971
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIA time-weighted average 0.1 ppm (0.52 mg/m3), Carcinogen, JUL2008
Occupational Exposure Limit-AUSTRIA TRK-TMW 0.02 ppm (0.1 mg/m3);KZW 0.08 ppm (0.4 mg/m3), skin, production, 2007
Occupational Exposure Limit-AUSTRIA TRK-TMW 0.04 ppm (0.2 mg/m3);KZW 0.16 ppm (0.8 mg/m3), skin, use and handl, 2007
Occupational Exposure Limit-BELGIUM time-weighted average 0.1 ppm (0.53 mg/m3), Skin, Carcinogen, MAR2002
Occupational Exposure Limit-DENMARK time-weighted average 0.01 ppm (0.05 mg/m3), skin, carc, MAY2011
Occupational Exposure Limit-FINLAND short term exposure limit 0.01 ppm (0.052 mg/m3), skin, NOV2011
Occupational Exposure Limit-FRANCE VME 0.1 ppm (0.5 mg/m3), C2 Carcinogen, FEB2006
Occupational Exposure Limit-HUNGARY ceiling concentration 0.1 mg/m3, Skin, Carcinogen, SEP2000
Occupational Exposure Limit-ICELAND time-weighted average 0.01 ppm (0.05 mg/m3), carc, skin, NOV2011
Occupational Exposure Limit-JAPAN Occupational Exposure Limit 0.1 ppm (0.52 mg/m3), skin, 2A carc, MAY2012
Occupational Exposure Limit-KOREA time-weighted average 0.1 ppm (0.5 mg/m3), skin, 2006
Occupational Exposure Limit-MEXICO time-weighted average 0.1 ppm (0.52 mg/m3), 2004
Occupational Exposure Limit-NEW ZEALAND time-weighted average 0.05 ppm (0.26 mg/m3), skin, JAN2002
Occupational Exposure Limit-NORWAY time-weighted average 0.01 ppm (0.05 mg/m3), JAN1999
Occupational Exposure Limit-PERU time-weighted average 0.1 ppm (0.52 mg/m3), JUL2005
Occupational Exposure Limit-POLAND MAC(time-weighted average) 0.5 mg/m3, MAC(short term exposure limit) 1 mg/m3, JAN1999
Occupational Exposure Limit-RUSSIA short term exposure limit 0.1 mg/m3, Skin, JUN2003
Occupational Exposure Limit-SWEDEN Group B Carcinogen, JUN2005
Occupational Exposure Limit-SWITZERLAND MAK-week 0.02 ppm (0.1 mg/m3), carc 2, skin, JAN2011
Occupational Exposure Limit-TURKEY time-weighted average 0.1 ppm (0.5 mg/m3), Skin, JAN1993
Occupational Exposure Limit-UNITED KINGDOM time-weighted average 0.05 ppm (0.26 mg/m3), skin, OCT2007
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction) 8H time-weighted average 1 ppm (5 mg/m3) (skin) CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors) 8H time-weighted average 1 ppm (5 mg/m3) (skin) CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry) 8H time-weighted average 1 ppm (5 mg/m3) (skin) CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards) 8H time-weighted average 1 ppm (5 mg/m3) (skin) CFRGBR 29,1915.1000,1993
NIOSH Documentation and Surveillance
Organization Standard Reference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO DIMETHYL SULFATE-air 8H Carcinogen time-weighted average 0.1 ppm (Sk) NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983 Hazard Code 26880; Number of Industries 5; Total Number of Facilities 185; Number of Occupations 23; Total Number of Employees Exposed 10481; Total Number of Female Employees Exposed 2455
National Occupational Hazard Survey 1974 Hazard Code 26880; Number of Industries 3; Total Number of Facilities 83; Number of Occupations 5; Total Number of Employees Exposed 1249
Status in Federal Agencies
Organization Reference
EPA GENETOX PROGRAM 1988, Inconclusive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Negative: D melanogaster-partial sex chrom. loss
EPA GENETOX PROGRAM 1988, Positive/dose response: In vitro SCE-human
EPA GENETOX PROGRAM 1988, Positive: Aspergillus-forward mutation; Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Positive: D melanogaster-reciprocal translocation
EPA GENETOX PROGRAM 1988, Positive: D melanogaster-whole sex chrom. loss
EPA GENETOX PROGRAM 1988, Positive: Host-mediated assay; E coli polA without S9
EPA GENETOX PROGRAM 1988, Positive: In vitro cytogenetics-nonhuman
EPA GENETOX PROGRAM 1988, Positive: In vitro SCE-nonhuman; D melanogaster Sex-linked lethal
EPA GENETOX PROGRAM 1988, Positive: S cerevisiae-reversion; S pombe-reversion
EPA TSCA 8(a) PRELIMINARY ASSESSMENT INFORMATION, FINAL RULE FEREAC 47,26992,82
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Dimethyl sulfate, 2524
NTP 14th Report on Carcinogens,2016:Reasonably anticipated to be human carcinogen
On EPA IRIS database
Page last reviewed: November 16, 2018
Content source: National Institute for Occupational Safety and Health
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