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        DPDx - Laboratory Identification of Parasites of Public Health Concern
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        Case #294 – February, 2011
          Case #294 – February, 2011
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                A 45-year-old woman, originally from Africa, presented to her primary care provider with diarrhea, nausea, vomiting, weight loss and gastritis. The symptoms occurred approximately three months post kidney transplant. Stool was collected in formalin and polyvinyl alcohol (PVA) for routine ova-and-parasite examination. A wet mount was made from the formalin-preserved stool and observed microscopically by the attending pathologist. Images were captured at 400x magnification and sent to the DPDx Team for diagnostic assistance. Figures A and B show two of the images received for evaluation. Size was not included in the initial inquiry. What is your diagnosis? Based on what criteria?
                    Figure A
                    Figure B
                  Case Answer
                        This was a case of strongyloidiasis, caused by Strongyloides stercoralis. The images showed first-stage rhabditoid (L1) larvae. Diagnostic morphologic features included:
                          * a short buccal canal (BC, Figure B).
                          * a prominent genital primordium (GP, Figure B).
                          * a rhabditoid esophagus (ES, Figure B).
                        Although a size was not given, the above features allowed for an accurate identification and to distinguish the organisms from hookworm larvae.
                        Figure B
                        More on: Strongyloidiasis
                This case and images were kindly provided by Boston Medical Center, Boston, MA.
                Images presented in the DPDx case studies are from specimens submitted for diagnosis or archiving. On rare occasions, clinical histories given may be partly fictitious.
      DPDx is an educational resource designed for health professionals and laboratory scientists. For an overview including prevention, control, and treatment visit www.cdc.gov/parasites/.
      Last Reviewed: August 24, 2016
        Source: National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of Parasitic Diseases and Malaria
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              o Detection of parasite antigens
              o Isolation of organisms
              o Special tests
              o Inactivation of Ebola/Marburg for malaria testing
          + Stool Specimens plus icon
              o Safety
              o Specimen Collection
              o Specimen Processing
              o Shipment
              o Staining
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              o Detection of parasite antigens
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              o Extraction of Parasite DNA from Fecal Specimens
              o Morphologic comparison of intestinal parasites
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              o Tissue specimens for free-living amebae(FLA)
              o Isolation of Leishmania organisms
              o Sputum
              o Aspirates
              o Sputum, induced sputum, and bronchoalveolar avage (BAL)
              o Vaginal swabs for diagnosis
              o Procedure for demonstration of pinworm eggs
              o Urine
              o Whole Organisms
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