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March 17, 2000 / 49(10);211
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                                                                                      Notice to Readers: Update: West Nile Virus Isolated from Mosquitoes --- New York, 2000                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                      During January--February 2000, three pools of overwintering Culex spp. mosquitoes collected at Fort Totten, New York, were positive for West Nile Virus (WNV) RNA by real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay (TaqMan™, Perkin-Elmer Biosystems, Foster City, California*) (1). Additional testing to detect the presence of intact virus was performed but was negative as of March 10, 2000. Since then, one of the pools that was positive by RT-PCR yielded a live WNV isolate in a fluid vero cell culture system and confirmed by West Nile-specific monoclonal antibody staining of virus infected cells and virus gene sequencing. Studies of this isolate continue.  
                                                                                      Reference                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                       1. CDC. Update: surveillance for West Nile Virus in overwintering mosquitoes---New York, 2000. MMWR 2000;49:178--9.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                      * Use of trade names is for identification only and does not imply endorsement by CDC or the U.S. Department of Health and Human Services.                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
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